Sağlıklı erişkinlerde fiziksel aktivitenin eritosit ve plazmadaki oksidan/antioksidan parametreler üzerine olan etkilerinin belirlenmesi ve yüksek antioksidan özelliği olduğu bilinen nar suyunun bu parametreler üzerine olan etkilerinin araştırılması
Özet
Several lines of studies demonstrated that physical activity, particularly strenuous exercise, results in oxidative stress, which may impair exercise performance and fatigue. Certain reports suggested beneficial role of antioxidant supplementation in exercise induced oxidative stress. Herein, we investigated influence of strenuous exercise on erythrocyte and plasma oxidant/ antioxidant parameters in professional football players. Efficacy of pomegranate juice on these parameters was studied as wellFifteen professional football players aged 18 to 35 and without any systemic illness and as a control group 15 healthy adults were enrolled into this study. Blood samples were obtained before and after in two different football match from study group. In the first match, no additional substance was given. In the second match, before and in the half-time period pomegranate was given to players. Blood samples were immediately obtained and separated to plasma and erythrocyte fractions. Malondialdehyte (MDA), catalase (CAT), peroxidase (GSH-Px) and superoxide dismutase (SOD), xantine oxidase (XO) and MDA, CAT, GSH-Px, SOD, XO were analyzed by biochemical methods in the erythrocytes and plasma samples respectively. İntercomparisons were made with Mann-Whitney U test and intracomparisons within groups were performed by Wilcoxon rank testsComparison of study group with control subjects revealed statistically significant increase in erythrocyte XO and decrease in GSH-Px (p<0,001 and p=0,021 respectively). Moreover plasma MDA was lower than that of control subjects in football players (p=0,001). No significant difference was observed in erythrocyte MDA, CAT, GSH-Px, SOD, and XO levels in samples taken before and after the match (p<0,05). Similarly, pomegranate juice did not affect these parameters. However MDA level of erythrocyte membrane was significantly decreased increased after ingestion of pomegranate (p=0.03). Plasma electrolytes values were significantly altered after the game with decrease in sodium, potassium, cloride and increase in calcium, phosphorus and lactate (p=0,001 for each). Pomegranate juice was significantly improved these electrolyte changes.Our results indicate that pomegranate juice would be of benefit in reducing detrimental effects of oxidative stress developing after physical activity. Beside, pomegranate juice markedly prevents electrolyte alterations in these individuals. Further studies are needed in order to clarify efficacy of pomegranate juice on exercise performance