HLA-G geninin 3'ut bölgesindeki 14 baz çiftlik delesyon polimorfizminin fertilizasyon üzerine etkisi
Özet
Why the maternal immune system does not reject the developing fetus remains a fundamental mistery of reproductive biology. The series of experiments presented here test the hypothesis that HLA-G antigen with limited polymorphism may be expressed in the human preimplantation embryos and immun regulation role and tolerogenic function of this gene was obtained. It is known that HLA-G molecule plays a crucial role in the implantation. In this study the efect of 14 bp deletion polymorphism of HLA-G gene on succesful implantation and pregnancy was examined. PCR method was preferred to detect this polymorphism. Patients, attending this study, were grouped into three: The first group (Group 1) as no pregnancy although at least three or more IVF treatment (n1=127), the second group (Group 2) succesful pregnancy after IVF treatment (n2=76) and the third group, fertil control group (n3=80) was described. Although there were differences between frequency of homozygote deletion, heterozygote deletion and non deleted conditions, no statistically significant difference was obtained (p>0.05). If we consider only the distribution of heterozygote women among each three group, an obvious difference for heterozygote rates was detected. As a result of this study, it was revealed that 14 bp deletion polymorphism of HLA-G did not affect IVF pregnancy.