Caco-2 epitel hücresi ve THP-1 makrofaj hücrelerinin Streptococcus pyogenes ve Escherichia coli ye karşı bakterisidal etki, nitrik oksit ve sitokin yanıtlarının araştırılması

Abstract

Epithelial cells and macrophages cells are believed to contribute to innate immune response by intercellular communications and secretion of inflammatory mediators. In this study, it was aimed to compare bactericidal capacity, NO and cytokine production of macrophages and epithelial cells alone, mixed and separated, against different bacteria. We found the mean values for bactericidal effects of Caco-2 cells and THP-1 cells alone, mixed and seperated by a filter insert as 21,96%, 27,73%, 24,36%, 31,83%, 27,55% on S. pyogenes and as 36,22%, 63,87%, 55,68%, 30,51%, 43,65% on E. coli, respectively. Our results showed a statistically significant increase in NO levels in epithelial and/or macrophage cell culture supernatants collected after 5 h exposure compared to control cells (p<0.05). When Caco-2 epithelial cells and THP-1 macrophages were incubated in vitro with S. pyogenes alone, mixed and separated for 5 and 24 hours, there were no statistically significant changes in the levels of cytokines except for IL-6 and TNF-? when the cells were mixed (p>0,05). On the other hand, cells which were stimulated with E. coli showed statistically higher levels of IL-6, IL-8 and TNF-? at 5 and 24 hours (p?0.05). Macrophages play a more active role than epithelial cells in bactericidal effect and cytokine response. Besides, epithelial cells and macrophages activate each other when mixed more than alone or separated.