Bacillus sphaerium suşlarının PAGE ile elde edilen hücredışı protein profillerine göre ayrımı
Özet
11 ABSTRACT Masters Thesis DISCRIMINATION OF Bacillus sphaericus STRAINS BY EXTRACELLULAR PROTEIN PROFILES OBTAINED BY PAGE Nazime MERCAN Ankara University Graduate School of Natural and Applied Sciences Department of Biology Supervisor: Assoc. Prof. Dr. Cumhur ÇÖKMÜŞ 1996, Page: 28 Jury: Assoc. Prof. Dr. Cumhur ÇÖKMÜŞ Prof. Dr. Şevki YAZGAN Assoc. Prof. Dr. Gülay ÖZCENGİZ In this study, a total of 19 strains of Bacillus sphaericus (13 of them pathogen, 6 of them nonpathogen) in both vegetative and sporulated stages were compared according to their filtrate protein profiles obtained by Native- PAGE and SDS-PAGE. When the strains were compared in sporulated stage, filtrate protein profiles obtained by native-PAGE differ the strains even in each phage and serogroups. On the other hand, generally it has been seen that the typing according to filtrate protein profiles was correlated with serotyping and phage typing. In addition, it has been understood that the discrimination of B. spha ericus strains by Native-PAGE was more useful. KEY WORDS: Bacillus sphaericus, Microbial control of mosquitoes, Classification, Electrophoresis.