Biberde (Capsicum annuum L.) kök gelişimiyle ilgili miyozin genlerinin tuzluluk stresi altında gen ifadesinin belirlenmesi

Abstract

Pepper (Capsicum annuum L.) is grown and consumed extensively and has high commercial value. Salinity stress negatively affects the growth, development and life of the plant. Roots take water and nutrients from the soil through their root hairs. Due to the increase in soil salt concentration, the water and nutrient intake through the roots decreases and toxic effects occur. Myosins are motor proteins that are involved in many physiological events in the plants. Studies investigating the genetic mechanism of root development linked them with the development of root and root hairs. In this study, gene expressions of myosin 2 (Capsicum annuum myosin-2-like), myosin 4 (Capsicum annuum myosin-4-like), myosin 6 (Capsicum annuum myosin -6-like), myosin 9 (Capsicum annuum myosin-9) and myosin 11 (Capsicum annuum myosin-11) and Capsicum annuum actin gene as the housekeeping gene were determined in root tissues of pepper seedlings 3 and 24 hours after 100 mM salt treatment. The highest increase in the expression was in myosin 9 gene (30.57 fold) 24 hours after salt treatment. The expression of myosin 2 gene did not show a statistically significant change 3 hours after salt treatment, however, it increased 8.95 fold after 24 hours. There was no significant change in the expression of the myosin 4 gene at both time points. Expression of the myosin 6 gene decreased -8.22 times after 3 hours of salt treatment, and -2.16 times after 24 hours. Myosin 11 gene expression decreased -3.53 times after 3 hours and it increased 2.35 times after 24 hours (P <0.05).