İç Anadolu bölgesindeki Tetranychid akarların (Acari: Tetranychidae) DNA barkodlaması ve Tetranychus urticae popülasyonlarının bazı akarisitlere karşı direnç durumlarının belirlenmesi
Özet
Spider mites (Acari: Tetranychidae) have been considered as the most economically important mite family in agricultural acarology. The most preferred method in spider mite management is chemical control all around the world. However, failure in chemical control of spider mites has often been reported because of both inaccurate species identification and resistance development against acaricides. In this study, molecular characterization of spider mite species have been performed using cytochrome oxidase I gene and internal transcribed spacer gene region. A total of 35 sequences for each gene belonging to 10 different species have been obtained and both gene sequences have been considered as suitable in the identification of spider mite species (with some exceptions). In addition, abamectin and pyridaben resistance and resistance mechanisms have been investigated in 10 Tetranychus urticae populations collected from Central Anatolia Region. 9-fold resistance ratio was determined among tested populations and all populations showed low resistance levels to abamectin. Up to 15-fold pyridaben resistance has been determined and it is expected that pyridaben application would fail at registered dose in more than half of the tested populations. Therefore, pyridaben should be used in rotation with other acaricide mode of action groups in these regions. In addition; alternative management methods, especially biological control, should be integrated in pest control programs. Previously reported target-site mutations have not been determined in T. urticae populations. The results of this study will contribute to both fast and accurate identification of tetranychid mites and create resistance management programs.