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dc.contributor.authorErgül, Ali
dc.contributor.otherNofouzi, Farzad
dc.contributor.otherOğuz, Muhammet Çağrı
dc.contributor.otherKhabbazi, Saber Delpasand
dc.date.accessioned2020-06-01T06:14:32Z
dc.date.available2020-06-01T06:14:32Z
dc.date.issued2019
dc.identifier.urihttps://doi.org/10.3906/tar-1804-52tr_TR
dc.identifier.urihttp://hdl.handle.net/20.500.12575/71270
dc.description.abstractAlfalfa is a fodder crop that accounts for one of the best sources of protein and is widely cultivated around the world. In vitro regeneration of alfalfa has been studied earlier; however, most of the studies were almost intervened with callus formation. In this study, 3 explant sources (cotyledonary node, hypocotyl, and root crown) of two Turkish cultivars (Nimet and Savaş) were excised from young seedlings. Explants were subjected to different concentrations of BAP, BAP–IBA, and TDZ to evaluate the direct in vitro regeneration potential of selected plant parts. Moreover, we transformed the alfalfa plant with pBin19 harboring 35s.GUS-INT_35s.nptII construct to investigate the transformation efficiency and regeneration frequency after bacterial inoculation. The transformation was carried out by Agrobacterium tumefaciens strain GV2260. The highest mean number of regenerated shoots per explant was recorded as 8.5, 6.66, and 6.33 shoots per explant after cotyledonary node explants were treated with BAP (0.40 mg/L), BAP–IBA (1.25–0.06 mg/L), and TDZ (0.55 mg/L), respectively. The highest gene transformation frequency was 9.52% and 6.19% based on PCR and GUS assays. The regeneration frequency was decreased by up to 48.1% under kanamycin selection pressure. The effect of cultivar on shoot regeneration frequency, mean number of regenerated shoots, and gene transformation efficiency was significant. This study contributes to in vitro regeneration of alfalfa crop and its genetic transformation which could be utilized in future gene transformation studies.tr_TR
dc.language.isoentr_TR
dc.relation.isversionof10.3906/tar-1804-52tr_TR
dc.subjectGene transformationtr_TR
dc.subjectGUS reporter systemtr_TR
dc.subjectPlant growth regulatortr_TR
dc.subjectShoot inductiontr_TR
dc.subjectTissue culturetr_TR
dc.titleImprovement of the in vitro regeneration and agrobacterium-mediated genetic transformation of medicago sativa Ltr_TR
dc.typeArticletr_TR
dc.relation.journalTurkish Journal of Agriculture and Forestrytr_TR
dc.contributor.departmentBiyoteknoloji Enstitüsütr_TR
dc.identifier.volume43tr_TR
dc.identifier.issue1tr_TR
dc.identifier.startpage96tr_TR
dc.identifier.endpage104tr_TR
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıtr_TR


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