Relation of Soluble Endothelial Protein C Receptor and Cytokines After Allogeneic Hematopoietic Stem Cell Transplantation
Aim: The objective of this study was to elucidate the effects of tumor necrosis factor a (TNF-a), interleukin 1b (IL-1b), interleukin 2 (IL-2), interleukin 6 (IL-6), and interleukin 8 (IL-8) on the expression of soluble endothelial protein C receptor (sEPCR) in the pathogenesis of thrombotic complications after hematopoietic stem cell transplantation (HSCT). Methods: The relationship between plasma concentrations of proinflammatory cytokines (TNF-a, IL-1b, IL-2, IL-6, and IL-8) and sEPCR was evaluated in 32 consecutive allogeneic hematopoietic stem cell–transplanted patients prior to conditioning regimen and randomly once between þ5 and þ30 days after transplantation and compared these results with 20 healthy controls. Results: Soluble endothelial protein C receptor levels did not indicate any significant difference between pre- and posttransplantation period, and sEPCR levels showed a significantly negative correlation between IL-6 and IL-8 (sEPCR and IL-6, r ¼ .43, P < .01; sEPCR and IL-8, r ¼ .57, P < .01). There was no correlation between sEPCR levels and TNF-a, IL-1b, or IL-2 (sEPCR and TNF-a, r ¼ .13, P > .05; sEPCR and IL-1b, r ¼ .1, P .05; sEPCR and IL-2, r ¼ .07, P > .05). Conclusions: Our results suggest that the production of sEPCR was not affected by allogeneic HSCT. Soluble endothelial protein C receptor did not show any positive correlation between these proinflammatory cytokines (TNF-a, IL-1b, IL-2, IL-6, and IL-8), on the contrary a significantly negative correlation was determined between sEPCR and either IL-6 or IL-8. This negative correlation may be a protective mechanism in the pathway of protein C activation.