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dc.contributor.authorÖzdag, Hilal
dc.contributor.otherSümer Çelebi, Hülya
dc.date.accessioned2020-04-29T07:14:58Z
dc.date.available2020-04-29T07:14:58Z
dc.date.issued2014
dc.identifier.urihttps://doi.org/10.3906/sag-1304-64tr_TR
dc.identifier.urihttp://hdl.handle.net/20.500.12575/71197
dc.description.abstractBackground/aim: Sensitive and cost-effective detection of point mutations is important in genetics research. Denaturing highperformance liquid chromatography (DHPLC) is known to be one of the most sensitive techniques for point mutation detection. A more recent technique, high-resolution melting (HRM), is based on the melting behavior of PCR products. In this study, the efficiency and sensitivity of HRM and DHPLC for the detection of MEFV, F5, and F2 gene point mutations were evaluated. Materials and methods: We studied 15 patients with MEFV mutations (E148Q, M680I, M694V, or V726A), 7 patients with the F51691G>A mutation, and 12 patients with the F220210G>A mutation. All mutations were screened by HRM and DHPLC. Results: All mutations were successfully detected by HRM. However, only 4 (MEFV E148Q and M680I, F51691G>A, and F220210G>A) of 6 mutations were successfully detected with DHPLC. Conclusion: Our study showed that HRM is more sensitive than DHPLC for detection of the studied point mutations. © TÜBİTAK.tr_TR
dc.language.isoentr_TR
dc.relation.isversionof10.3906/sag-1304-64tr_TR
dc.subjectDenaturing high-performance liquid chromatographytr_TR
dc.subjectHigh-resolution melting analysistr_TR
dc.subjectPoint mutationstr_TR
dc.titleComparison of high-resolution melting analysis to denaturing high performance liquid chromatography in the detection of point mutations in MEFV, F5, and F2 genestr_TR
dc.typeArticletr_TR
dc.relation.journalTurkish Journal of Medical Sciencestr_TR
dc.contributor.departmentBiyoteknoloji Enstitüsütr_TR
dc.contributor.authorIDhttp://orcid.org/0000-0001-7940-2499tr_TR
dc.identifier.volume44tr_TR
dc.identifier.issue5tr_TR
dc.identifier.startpage713tr_TR
dc.identifier.endpage719tr_TR
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıtr_TR
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dc.description.indexScopus


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