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dc.contributor.advisorKınıklı, Sami
dc.contributor.authorKınıklı, Gülay
dc.contributor.otherTürkçapar, Nuran
dc.contributor.otherKucukay, Murat Bulent
dc.contributor.otherKeskin, Göksal
dc.date.accessioned2020-03-26T07:10:49Z
dc.date.available2020-03-26T07:10:49Z
dc.date.issued2005
dc.identifier.urihttps://doi.org/10.1016/j.diagmicrobio.2005.02.016tr_TR
dc.identifier.urihttp://hdl.handle.net/20.500.12575/70761
dc.description.abstractThe aim of the present study was to determine the cellular immune response on the course of brucellosis by investigating the proliferation response of T-cell subsets to phytohemagglutinin (PHA), that is, nonspecific mitogen in the patients with acute and chronic brucellosis. The study was performed in 19 patients with untreated brucellosis (acute, n = 11; chronic, n = 8) and 19 healthy controls. Standard tube agglutination and Coombs tests for brucellosis were performed. CD4+ and CD8+ T cell were investigated by the flow cytometry and sorting methods in all of cases. After these cells were cultured and stimulated with PHA, [H3]-thymidine uptake and stimulation indices (SIs) were established. In all of the patients with brucellosis, CD4+ SIs and CD8+ SIs were found to be 1.40 ± 0.63 and 1.45 ± 0.42, respectively, and in the controls CD4+ SIs and CD8+ SIs were 1.59 ± 0.36 and 1.64 ± 0.37, respectively. In acute cases, CD4+ SIs were 1.71 ± 0.64 and CD8+ SIs were 1.54 ± 0.45. CD4+ SIs were 0.97 ± 0.25 and CD8+ SIs were 1.32 ± 0.37 in chronic cases. Although in acute cases CD4+ SIs and CD8+ SIs were not different from those in the control group, CD4+ SIs of chronic brucellosis cases were found to be significantly low as compared with those of acute brucellosis cases and the controls (P < 0.01). CD8+ SIs of acute and chronic brucellosis cases were not found to be different from those in the controls. Brucella agglutination titers of the patients with acute and chronic brucellosis were not found related with CD4 SIs and CD8 SIs. The findings of significantly low results of CD4+ T-cell proliferative responses of chronic brucellosis to PHA as compared with control and acute brucellosis cases remind that the development of chronic infection might be a result of T-helper proliferation defect. © 2005 Elsevier Inc. All rights reserved.tr_TR
dc.language.isoentr_TR
dc.relation.isversionof10.1016/j.diagmicrobio.2005.02.016tr_TR
dc.subjectBrucellosistr_TR
dc.subjectT-cell subsetstr_TR
dc.subjectCellular immune responsetr_TR
dc.subjectImmunitytr_TR
dc.titleIn vitro nonspecific mitogenic response of T-cell subsets in acute and chronic brucellosistr_TR
dc.typeArticletr_TR
dc.relation.journalDiagnostic Microbiology and Infectious Diseasetr_TR
dc.contributor.departmentTıp Fakültesitr_TR
dc.identifier.volume52tr_TR
dc.identifier.issue3tr_TR
dc.identifier.startpage229tr_TR
dc.identifier.endpage233tr_TR
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıtr_TR


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