The ınhıbıtory effect of benzodıazepıne derıvatıves on the bovıne lens aldose reductase enzyme.

Abstract

Summary. The aldose reductase enzyme, involved in the sorbitol pathway which is an important mechanism in regulation of mammalian glucose metabolism, has been known to play a significant role in the initiation of diabetic complications. Numerous chemical substances have been prepared in order to improve the pharmacological profile of inhibition of aldose reductase enzyme. In this study, aldose reductase inhibitor,/ activities of several benzodiazepine derivatives were investigated. The enzyme was obtained from bovine lenses via the ammonium sulphate-protein cut method with several steps. It was found that tetrazepam had a significant inhibitor potency among the other benzodiazepine derivatives showing very slight inhibitor activities that are indicated in terms of percent inhibitor potency at 10 -4 M concentration.