Sıçanlarda kafes yoğunluğunun bazı önemli özelliklere etkileri
Özet
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mso-para-margin-top:0cm; mso-para-margin-right:0cm; mso-para-margin-bottom:10.0pt; mso-para-margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-fareast-language:EN-US;} <p class="MsoNormal" style="margin-bottom:0cm;margin-bottom:.0001pt;line-height: normal;mso-layout-grid-align:none;text-autospace:none"><span style="font-family: "Arial","sans-serif"">Bu çalışma laboratuvar sıçanlarının barındıkları kafeslerde hayvan yoğunluğu ve hayvan başına düşen yaşama alanının döl verimi, canlı ağırlık ve bağışıklık gücüne etkisini ortaya koymak amacıyla yapılmıştır. <p class="MsoNormal" style="margin-bottom:0cm;margin-bottom:.0001pt;line-height: normal;mso-layout-grid-align:none;text-autospace:none"><span style="font-family: "Arial","sans-serif"">Araştırma, Sağlık Bakanlığı Ankara Eğitim ve Araştırma Hastanesi Deney Hayvanları Laboratuvarında yürütülmüştür. Araştırmanın hayvan materyalini Wistar Albino ırkına ait, 6 haftalık yaşta 65 dişi ve 25 erkek olmak üzere toplam 90 adet sıçan oluşturmuştur. <p class="MsoNormal" style="margin-bottom:0cm;margin-bottom:.0001pt;line-height: normal;mso-layout-grid-align:none;text-autospace:none"><span style="font-family: "Arial","sans-serif"">Araştırma konusu 2 farklı deneme halinde ele alınmıştır. Birinci denemede kafes yoğunluklarının etkisini araştırmak amacıyla, her kafeste hayvan başına 194.4 cm<span style="font-size:7.0pt;font-family: "Arial","sans-serif"">2 alan düşecek şekilde kafesler düzenlenmiştir. Eş zamanlı ikinci denemede ise her grupta eşit sayıda (2 dişi, 1 erkek) sıçan bulundurulmak koşuluyla yine 3 grup oluşturulmuş, ama bu gruplarda hayvan başına düşen yaşama alanı normal(194.4cm2/sıçan), dar (155.2cm2 /sıçan) ve geniş (259.2cm2 /sıçan) olacak <p class="MsoNormal" style="margin-bottom:0cm;margin-bottom:.0001pt;line-height: normal;mso-layout-grid-align:none;text-autospace:none"><span style="font-family: "Arial","sans-serif"">şekilde ayarlanmıştır. <p class="MsoNormal" style="margin-bottom:0cm;margin-bottom:.0001pt;line-height: normal;mso-layout-grid-align:none;text-autospace:none"><span style="font-family: "Arial","sans-serif"">Döl verimi yönünden I.denemede yavrulama büyüklüğü ( bir doğumda elde edilen toplam yavru sayısı ) gruplar arasında sırasıyla 7.95; 7.84; 8.58 adet ve canlı doğan yavru sayısı 6.96; 7.78; 8.42 adet ve yaşama gücü % 84.9; 94.1;98.1 (P<0.001) ve sütten kesilen yavru sayısı 4.58; 5.60; 6.32 adet ve sütten kesim yaşama gücü % 52.1; 58.3; ve 59.4 (P>0.05)olarak bulunmuştur. II.denemede döl verimi; yavrulama büyüklüğü gruplar arasında sırasıyla 8.58; 9.12; 8.54 adet ve canlı doğan yavru sayısı 8.42; 8.65; 7.79 adet ve yaşama gücü 98.1; 94.9; 91.2 (P<0.01) ve sütten kesilen yavru sayısı 6.32; 5.80; 6.11adet ve yaşama gücü % 59.4; 51.6; 53.5 olarak bulunmuştur. <p class="MsoNormal" style="margin-bottom:0cm;margin-bottom:.0001pt;line-height: normal;mso-layout-grid-align:none;text-autospace:none"><span style="font-family: "Arial","sans-serif"">Canlı ağırlık yönünden I.denemede doğum ağırlıkları gruplar arasında sırasıyla 4.97; 4.90; 4.91 g ve sütten kesimde ağılıkları 36.29; 29.47; 31.6g (P<0.001) olarak bulunmuştur. II. denemede doğum ağırlıkları gruplar arasında sırasıyla 4.92; 4.98; 5.04 g ve sütten kesimde ağılıklar 31.62; 27.90 ve 26.08 g olarak bulunmuştur. <p class="MsoNormal" style="margin-bottom:0cm;margin-bottom:.0001pt;line-height: normal;mso-layout-grid-align:none;text-autospace:none"><span style="font-family: "Arial","sans-serif"">Bağışıklık düzeyleri (SRBC) I. denemede gruplarda deneme başında gruplarda sırasıyla 3.94; 5.62; 5.43 (P<0.01) ve deneme sonunda 4.24; 4.92; 3.86 olarak bulunmuştur. II.denemede deneme başında sırasıyla 5.00; 5.43; 6.29 (P<0.001)(P<0.01) 3.56; 3.86 ve 3.86 olarak bulunmuştur. <p class="MsoNormal" style="margin-bottom:0cm;margin-bottom:.0001pt;line-height: normal;mso-layout-grid-align:none;text-autospace:none"><span style="font-size:12.0pt;font-family:"TimesNewRoman,Bold","serif";mso-bidi-font-family: "TimesNewRoman\,Bold"">Summary <p class="MsoNormal" style="margin-bottom:0cm;margin-bottom:.0001pt;line-height: normal;mso-layout-grid-align:none;text-autospace:none"><span style="font-family: "Arial","sans-serif"">This study aims to show the effects of cage density and cage space on reproduction traits and immune system of laboratory rats. <p class="MsoNormal" style="margin-bottom:0cm;margin-bottom:.0001pt;line-height: normal;mso-layout-grid-align:none;text-autospace:none"><span style="font-family: "Arial","sans-serif"">The research has been conducted in Ministry of Health, Ankara Experiment was carried out in Education and Research Hospital’s Test Animals Laboratory. Totally 90 Wistar albino rats, which were 6 weeks of age, were used. 65 of them were female and 25 of them were male. There were two different experiments. In the first experiment the aim was to investigate the effect of cage density. So that each cage was created to provide 194.4 cm2 cage space per rat. In the concurrent second experiment there were same numbers of rats in each cage (2 females, 1 male) but in these groups the cage space per animal was arranged as normal (194.4cm2/rat), narrow (155.2cm2 /rat) and large (259.2cm2 /rat). <p class="MsoNormal" style="margin-bottom:0cm;margin-bottom:.0001pt;line-height: normal;mso-layout-grid-align:none;text-autospace:none"><span style="font-family: "Arial","sans-serif"">In the first experiment reproduction results among groups ; litter size were 7.95; 7.84; 8.58 and alive litters were 6.96; 7.78; 8.42, survival rate was % 84.9; 94.1;98.1 (P<0.001), weaned litters were 4.58; 5.60; 6.32 and survival rate of weaned litters was found to be 52.1; 58.3; ve 59.4(P>0.05) in numbers respetively. In the second experiment reproduction results ;litter size 8.58; 9.12; 8.54, alive litters were 8.42; 8.65; 7.79 survival rate was 98.1; 94.9; 91.2 (P<0.01), weaned litters were 6.32; 5.80; 6.11 and survival rate of weaned litters were % 59.4; 51.6; 53.5 in numbers respectively. <p class="MsoNormal" style="margin-bottom:0cm;margin-bottom:.0001pt;line-height: normal;mso-layout-grid-align:none;text-autospace:none"><span style="font-family: "Arial","sans-serif"">In the first experiment birth weights among groups were 4.97; 4.90; 4.91 g and weights at weaning were 36.29; 29.47; 31.62g (P<0.001) respectively. In the second experiment the birth weights were 4.92; 4.98; 5.04 g and weaning weights were 31.62; 27.90 ve 26.08 g respectively. <p class="MsoNormal" style="margin-bottom:0cm;margin-bottom:.0001pt;line-height: normal;mso-layout-grid-align:none;text-autospace:none"><span style="font-family: "Arial","sans-serif"">Immune levels (SRBC) were 3.94; 5.62; 5.43 (P<0.01) among groups <p class="MsoNormal" style="margin-bottom:0cm;margin-bottom:.0001pt;line-height: normal;mso-layout-grid-align:none;text-autospace:none"><span style="font-family: "Arial","sans-serif"">respectively at the beginning of the first experiment and were 4.24; 4.92; 3.86 respectively at the end of the first experiment. At the beginning of the second experiment the immune levels were 5.00; 5.43; 6.29 (P<0.001) respectively and at the end they were as (P<0.01) 3.56; 3.86 ve 3.86 respectively.