Aytekin, İbrahimFadhıl, Marwan2022-09-132022-09-132022https://doi.org/10.15832/ankutbd.747211http://hdl.handle.net/20.500.12575/83978The aim of this study is to determine the genetic variation of the Pit 1 gene and comparison polymorphisms between four cattle breeds (Anatolian Black, Holstein Friesian, Brown Swiss, and Simmental). A total of two hundred animals were used for this purpose in four different cattle breeds, each with 50 heads. Genetic variations between breeds were identified via RFLP method by PCR. The allele frequency A and B for Anatolian Black, Holstein Friesian, Brown Swiss, and Simmental was 0.260, 0.740; 0.320, 0.680; 0.100, 0.900; 0.230, 0.770 respectively. While the genotype frequency AA, AB and BB for Anatolian Black, Holstein Friesian, Brown Swiss, and Simmental was 0.10, 0.32 and 0.62; 0.10, 0.44 and 0.46; 0.00, 0.20 and 0.80; 0.10, 0.26 and 0.64 respectively. According to the chi-square test, all breeds were found to be in Hardy–Weinberg equilibrium (P>0.05). As a result, it can be said that with a more comprehensive study that will include economic traits in these breeds, revealing association analyses would be more informative in the future.enPit-1Genetic variationsHardy–Weinberg equilibriumArticle28011391442148-9297