Bazı Lactarius türlerinin morfolojik, anatomik özellikleri ve protein bantlarının karşılaştırılması
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1999
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Fen Bilimleri Enstitüsü
Abstract
In this study, the morphological and microscopical properties of the specimens from the species, Lactarius deliciosus, Lactarius sanguifluus, Lactarius semisanguifluus,Lactarius deterrimus, Lactarius salmonicolor belonging to Lactarius genus, Russulaceae family and Russulales order were examined and also protein profiles of these specimens were analysed by PAGE. In this investigation, morphological similarities and differences of these species were determined both by botanical excursions and by laboratory studies. Microscopical examination of cuticle and spores were done.According to structure of cuticle, it was observed that ; Lactarius deliciosus, Lactarius deterrimus and Lactarius salmonicolor are belong to monomitic hyphal system structure Lactarius sanguifluus and Lactarius semisanguifluus are belong to dimitic hyphal system structure. Both SDS - PAGE and N - PAGE analysis were demonstarted that these species have different protein band profiles. The five species which the morphological properties are very similar, were identified by the structure of their cuticle and also shown by PAGE analysis that they are completely different species from the view of molecular typing. Key Words : Lactarius, mushrooms, classification, morphology, anatomy, protein, electrophoresis
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